Affiliations: NEC Laboratories America, Inc., Princeton, NJ, USA | Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
Note: [] Corresponding author: Anurag Sharma, PICT Lab, Massachusetts General Hospital, 101 Merrimac Street, Ste 820, Boston, MA 02114, USA. Tel.: +1 617 643 7913; E-mail: [email protected]
Abstract: The objective is to find a practical balance between quality and performance for daily high volume whole slide imaging. We evaluated whole slide images created by various scanners at different compression factors to determine the best suitable quality factor (QF) needed for pathological images of special stains. Method: We scanned two sets of eight special stains slides each at 0.50 μm/pixel resolution in Hamamatsu scanner at six and five QF levels respectively to generate 72 images which were observed at a calibrated monitor by imaging specialists, a histo-technician, and a pathologist to find the most suitable QF level for special stains in digital slides. Results: Most special stains images were acceptable at QF 30 except for the stain Reticulin where the lowest acceptable QF was 50. The compression of images from QF 90 to QF 50 reduced the size of the images by 62.73%. Conclusion: 0.50 μm/pixel images at QF 50 or above were found suitable 12 special stain.
Keywords: Special stains, image, quality factor, compression, whole slide, image quality, digital pathology
