Journal of Cellular Biotechnology - Volume 8, issue 2
Purchase individual online access for 1 year to this journal.
Price: EUR N/A
Journal of Cellular Biotechnology is a peer-reviewed international journal for advancing research activities in the field of cellular biotechnology. It serves as a medium for the publication of full papers, invited reviews, short communications, technical notes and letters to the Editor-in-Chief on all aspects of cellular biotechnology. This comprises molecular biological topics covering biochemical, chemical, pharmacological or bioprocess engineering aspects, as well as the development of novel biomaterials. Therefore, cellular biotechnology differs from biology, biochemistry, and other basic life sciences by its emphasis on using the knowledge of bioscience to solve important practical problems. Papers presenting information of a multidisciplinary nature - not suitable for publication in a journal devoted to a single discipline - are particularly welcome.
Manuscripts submitted for the
Journal of Cellular Biotechnology are expected to cover activities related to molecular diagnostics, the expansion of human primary cells for individualized therapies or drug testing, 2- and 3-dimensional co-culture techniques, cell line validation, tissue engineering, and stem cell biology for the treatment of human pathologies. This includes studies on the design of reactors and research on cellular biology and physiology of mammalian cells in vitro and in vivo, and tissue. Of special interest is the rational manipulation of reactions through metabolic engineering techniques or specific reactor operations that lead to biomaterials with unique properties. Also, biochemical and physiological studies of metabolism and enzymes as relevant for tissue culture cells, investigations at the molecular level including transcription/translation control; design and engineering of products by molecular strategies; engineering of cellular modification and transport systems such as post-translational protein modifications as well as protein and metabolite secretion; molecular strategies of screening for new or modified products (e.g. pharmaceuticals or bioactive compounds). In addition, investigations in preclinical animal experiments are welcome.
The endeavour of the Editor-in-Chief and publisher of the
Journal of Cellular Biotechnology is to bring together contributions from those working in various fields related to cell-cell or cell-material interactions all over the world. The editorial board members of the
Journal of Cellular Biotechnology are from those countries in Europe, Asia, Australia and America where appreciable work in cellular biotechnology is being carried out. Each editor takes responsibility to decide on the acceptance of a manuscript. He/she is required to have the manuscript appraised by two referees and may be one of them himself. The executive editorial office, to which the manuscripts have been submitted, is responsible for rapid handling of the reviewing process.
Abstract: BACKGROUND: TNF-α (Tumor necrosis factor-alpha) plays a vital role in the human immune system. However, abnormal activity and overexpression of TNF-α are some of the causes of allergic and autoimmune diseases. Inhibiting the activity of this molecule is one of the novel pathologies for these diseases. The development of this recombinant protein is expected to reduce the financial burden of treating inflammatory rheumatic diseases. OBJECTIVE: The study’s objective was to generate and evaluate the biological activity of TNFR-Fc, construct of fusions an extracellular part TNF-α receptor (p75) and an Fc fragment of human immunoglobulin G1, expressed from the…CHO-DG44 cell system. METHODS: The recombinant TNFR- Fc plasmid was constructed and identified by PCR, restriction enzyme digestion, and sequencing. A stable cell line for expression of TNFR-Fc was selected by limiting dilution cloning. Structural characterization, the binding affinity of TNFR-Fc to TNF-α, the neutralizing the cytotoxic activity- induced TNF-α, and the TNF-α-induced apoptosis suppression of TNFR- Fc were analyzed by SDS/PAGE Western blotting, ELISA, WST assay, Immunofluorescence, and flow cytometry. RESULTS: Preliminary analysis of the structural characteristics showed that TNFR-Fc is a low- glycosylated protein and perhaps in dimeric form. Furthermore, the recombinant TNFR-Fc can interact with its ligand TNF-α with a dissociation constant Kd 0.25±0.03μM equivalent to that of the original drug, Etanercept. We also demonstrated that TNFR-Fc expressed from CHO-DG44 was able to neutralize TNF-α- induced cytotoxic activity and inhibited p53-related apoptosis in vitro , similar to Etanercept. CONCLUSIONS: These data collectively suggested that TNFR-Fc potently blocks TNF-α, which could be a novel therapeutic strategy for cytokine-driven diseases.
Abstract: Reactive oxygen species (ROS) are free radical molecules obtained from biotransformation of molecular oxygen. Oxidative stress is referred as oxidative damage produced by free radicals, and has been associated with many diseases including periodontitis. Periodontitis is a chronic inflammatory disease resulting in loss of attachment and bone loss. There is over production of ROS in periodontitis, imbalance between free radicals and antioxidants locally result in destruction of periodontal tissues. Flavonoids are naturally occurring compounds known for various actions, such as, anti-inflammatory, antioxidant properties. The objective of this review is to provide an outline on the biological actions of flavonoids and…their beneficial effects on periodontal tissues.
Abstract: Cancer is the second most common reason for death in the world. The cancer research over four decades has been reached to the prospective on dysregulation of ions like (Ca2+ , Mg2+ , Na + , K +, or Cl - ) recently. These ions are orchestrated through numerous proteins, ion channels, selectively or non-selectively. However, the dysregulation of these ions and their channel expression are being reported for various diseases but here we have reviewed precisely TRP channels (TRPC and TRPM) for their role in cancer. The transient receptor potential (TRP) channels were first discovered in Drosophila melanogaster in 1989…and since then the superfamily becomes a group of 30 members under six subsections. Interestingly, we found that the TRPC (Canonical) channels, with 6 members, were explored in nine different types of cancers in last two decades. Additionally, we included the TRPM (Melastatin) subfamily and reviewed their role in cancer. Conclusively, these studies support that TRP channel-based therapies must be taken forward for clinical studies. Some channels, such as TRPC6, TRPM7 and TRPM8 were explored extensively in many cancer types which may be a potential target for cancer treatment. However, TRPM8 in lung cancer was reported for reverse association with cell proliferation, which needs to be reverified in lung cancer and other cancers. Besides, some TRPC channels are associated with store-operated calcium entry (SOCE) such as TRPC1, TRPC4 and TRPC6. Interestingly, the TRPC6 role was reported in breast cancer for modulation of Ca2+ through translocation of Orai1 and Orai3.
Keywords: Ion channel, TRP channels, Cancer, Migration, acidic extracellular pH
Abstract: BACKGROUND: Semen analysis is a routine predictor of male fertility, and however, measurements of sperm morphology, motility, and concentration do not always evince genomic defects. OBJECTIVE: To investigate sperm parameters of renal transplant patients and to evaluate sperm DNA defects. METHODS: Seminal samples from 25 healthy controls and 56 transplantation patients were analyzed to evaluate DNA fragmentation by TUNEL. The differences in TUNEL-assay results and seminal parameters were compared between kidney transplant patients and controls. RESULTS: Among the azoospermic patients, 37.5% had fathered children before the disease. Three patients receiving sirolimus treatment had oligoasthenoteratozoospermia…and infertility. In kidney transplant patients, DNA fragmentation was slightly higher than controls. Total motility (%) of the spermatozoa from the kidney transplant patients (42.2±21.9) was significantly lower (P < 0.05) than those of the control group (64.3±11.9). Moreover, control individuals had significantly higher (P < 0.05) normal morphology (23.2%) compared to the patient group (20.3%). Concerning sirolimus treatment, three patients had severe oligoasthenoteratozoospermia in their ejaculate, and however, DNA fragmentation rates were not significantly higher than those in the remaining individuals of the transplant group. CONCLUSIONS: The sperm DNA fragmentation rate in kidney transplant patients was slightly higher than in the control group (P = 0.09). However, the amount of spermatozoa DNA damage may lead to infertility in kidney transplant patients.
Abstract: Tissue engineering comprises of an array of specialities which combines biology, chemical sciences, engineering and material sciences for the regeneration of diseased tissues. In the novel world of tissue engineering, the fabrication and role of scaffolds is vital. Scaffolds have been engineered in such a fashion that it causes the desirable cellular interactions for the formation of new tissues for medical purposes. Ideal characteristics of scaffold include; three –dimensional and highly porous, should be biocompatible and bioresorbable, should have suitable surface chemistry for cell attachment, proliferation, and differentiation and must have mechanical properties to match those of the tissues at…the site of implantation. A high porosity and an adequate pore size are necessary to facilitate cell seeding and diffusion throughout the whole structure of both cells and nutrients. The ever- evolving world of medical science will now in the near future be able to regenerate the lost tissues with the advancements of tissue engineering.
Abstract: BACKGROUND: Scaling and root planing [SRP] being the mainstay of treatment of periodontitis encompasses unambiguous impediments. antiseptics represent an aid to nonsurgical periodontal therapy. OBJECTIVE: This randomized controlled, split mouth study design with an observation period of three months aims to clinically evaluate the efficacy of ozonised oil and chlorhexidine as an adjunct to SRP. METHODS: Twenty-five patients of both sexes with an age range of 30–65 years diagnosed with chronic periodontitis and having a periodontal probe depth (PD)≥5 mm and CAL≥3 mm on at least 1 site in each quadrant were included in this randomised split mouth…design study. Patients were allocated in 2 experimental treatment groups as SRP + chlorhexidine gel (control sites) and with SRP + ozone oil (test sites). The plaque index (PI), gingival index (GI), and periodontal pocket depth (PPD), clinical attachment level (CAL) were recorded at baseline data and after 30 days post-baseline. RESULTS: The present study showed significant results in both the groups with regards to the improvement in the clinical parameters. When comparison was made between the two groups, it has been assessed that the use of the ozonized oil in addition to SRP did not show significant differences when compared to conventional SRP + chlorhexidine. CONCLUSION: For bye to SRP, ozonized oil can be considered as a viable alternative to chlorhexidine in the treatment of periodontitis, especially considering its low toxicity compared to chlorhexidine.