Journal of Cellular Biotechnology - Volume 8, issue 1
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Journal of Cellular Biotechnology is a peer-reviewed international journal for advancing research activities in the field of cellular biotechnology. It serves as a medium for the publication of full papers, invited reviews, short communications, technical notes and letters to the Editor-in-Chief on all aspects of cellular biotechnology. This comprises molecular biological topics covering biochemical, chemical, pharmacological or bioprocess engineering aspects, as well as the development of novel biomaterials. Therefore, cellular biotechnology differs from biology, biochemistry, and other basic life sciences by its emphasis on using the knowledge of bioscience to solve important practical problems. Papers presenting information of a multidisciplinary nature - not suitable for publication in a journal devoted to a single discipline - are particularly welcome.
Manuscripts submitted for the
Journal of Cellular Biotechnology are expected to cover activities related to molecular diagnostics, the expansion of human primary cells for individualized therapies or drug testing, 2- and 3-dimensional co-culture techniques, cell line validation, tissue engineering, and stem cell biology for the treatment of human pathologies. This includes studies on the design of reactors and research on cellular biology and physiology of mammalian cells in vitro and in vivo, and tissue. Of special interest is the rational manipulation of reactions through metabolic engineering techniques or specific reactor operations that lead to biomaterials with unique properties. Also, biochemical and physiological studies of metabolism and enzymes as relevant for tissue culture cells, investigations at the molecular level including transcription/translation control; design and engineering of products by molecular strategies; engineering of cellular modification and transport systems such as post-translational protein modifications as well as protein and metabolite secretion; molecular strategies of screening for new or modified products (e.g. pharmaceuticals or bioactive compounds). In addition, investigations in preclinical animal experiments are welcome.
The endeavour of the Editor-in-Chief and publisher of the
Journal of Cellular Biotechnology is to bring together contributions from those working in various fields related to cell-cell or cell-material interactions all over the world. The editorial board members of the
Journal of Cellular Biotechnology are from those countries in Europe, Asia, Australia and America where appreciable work in cellular biotechnology is being carried out. Each editor takes responsibility to decide on the acceptance of a manuscript. He/she is required to have the manuscript appraised by two referees and may be one of them himself. The executive editorial office, to which the manuscripts have been submitted, is responsible for rapid handling of the reviewing process.
Abstract: BACKGROUND: Wound healing needs to occur after injury to prevent vision loss. Models of wound healing need to be optimized to assure treatments for corneal wounds can be developed in vitro prior to investigating with in vivo studies. OBJECTIVE: The purpose of this study was to establish the optimum media to use as a control solution in wound healing models. METHODS: Immortalized human corneal epithelial cells were cultured in different growth media using a scratch and exclusion zone model. The effect of normoxic and hypoxic conditions on tight junctional integrity and metabolic activity of…cells grown in different growth medium were also investigated. RESULTS: Wound healing with DMEMF12 media was significantly faster than both Keratinocyte serum-free media (p < 0.05) and EpiLife (p < 0.05) after 10 hours recovery under normoxic or hypoxic conditions using the scratch model and 9 days after wounding using the exclusion zone technique (p < 0.05). Using the culture media DMEMF12, cells stained for abundant ZO-1, Cx43 and had a high metabolic activity indicating significant epithelial barrier formation, gap junction formation and high cell viability. CONCLUSIONS: DMEMF12 led to superior wound healing under hypoxic and normoxic conditions and in two different wound healing models.
Abstract: Various acute phase reactants are produced in response to inflammatory stimuli, one of them is C-reactive protein. Serum levels of C-Reactive protein are useful to monitor and diagnose inflammatory process associated with disease as it being one of the valuable biomarkers in various clinical conditions, increase in level of C-Reactive protein is found in subjects with periodontal disease, which in turn can act as useful biomarker to diagnose cardiovascular disease, adverse pregnancy outcome as they being associated with periodontal disease. This manuscript is brief overview of C-Reactive protein and its relation with periodontal disease and systemic health.
Keywords: C-reactive proteins, periodontal disease, systemic health
Abstract: Tissue engineering aims to reconstruct the natural target tissue by a combination of three key elements stem/progenitor cells (that will create the new tissue), signaling molecules (that instruct the cells to form the desired tissue) scaffold/extracellular matrix (to hold the cells). Regeneration of the periodontal tissues following destructive episodes of various forms of periodontitis is a formidable challenge to periodontologists. Bone morphogenic proteins have been considered as the most potent growth factors that can promote the bone regeneration. This review will emphasize on the unique nature of the tissue engineered bone morphogenic proteins molecules regarding their structure, classification, signaling mechanism,…etc. which will further help in understanding their role and potential advances necessary to facilitate the process of regeneration in the field of periodontics.
Keywords: Bone morphogenic
proteins, periodontics, regeneration, tissue engineering
Abstract: BACKGROUND: Butyrylcholineesterase (BChE) is a therapeutic drug and its producing as a recombinant protein is an essential issue in biotechnology. One of the highlights in this regard is choosing the best host cells and plasmids. OBJECTIVES: The aim of this study is to evaluate the production of butyrylcholinesterase in Vero, HEK-293, and CHO cell lines using a dual promoter vector. MATERIAL AND METHODS: The dual-promoter construction (pBudCE dual BChE) was transfected into cell lines categorized in three experimental groups (pBudCE dual BChE, pCMV and negative control). BChE gene expression and enzyme activity was evaluated at different…times. RESULTS: All three cell lines showed higher gene expression level in pBudCE dual BChE group. BChE enzyme activity level of this group in CHO cells decreased in sixth day and increased in ninth day. In HEK-293 cells it has a downward trend from sixth to ninth day and in Vero cells its level in the ninth day was the highest. CONCLUSION: The difference of pBudCE dual BChE and pCMV groups was more pronounced in the HEK-293 cell and the BChE gene expression level of this cells was higher than the others while, CHO cells showed higher level of BChE enzyme activity.
Keywords: Butyrylcholinesterase, organophosphorus poisoning, dual-promoter vector, HEK-293, CHO, Vero
Abstract: The currently prevalent COVID-19 infection, its line of treatment, resultant immunosuppression, and pre-existing comorbidities have made patients exposed to secondary infections including mucormycosis. Mucormycosis is a rare but in invasive fungal infection (IFI) due to several species of saprophytic fungi, occurring in patients with underlying co-morbidities which include diabetes mellitus, organ transplant, immunosuppressive corticosteroid therapy. The maxilla rarely undergoes necrosis due to its rich vascularity. Rare but not uncommon is the incidence of mucormycosis associated maxillary osteomyelitis occurring post COVID-19 infection. Fungal osteomyelitis is a life-threatening infection which may further spread from maxilla to the nose and paranasal sinuses within…the orofacial region. It is an aggressive infection that needs to be addressed promptly to prevent fatal consequences.
Abstract: Platelet aggregates has been used in surgery for many years. The primary goal of these ‘Autologous Preparation’ was to aggregate platelets and their growth factors and to convey it to a surgical site, to improve healing only. The latest research work shows that the PRF also can be used for Protection of Graft, neo-angiogenesis, vascularization locally and release of various growth factors apart from healing. Platelet rich fibrin (PRF) is considered as the new generation of platelet concentrate. “Choukroun’s PRF” or Leucocyte PRF (L-PRF) is the new development, which was first developed by Choukroun’s et al. in 2001 as…an ‘Autologous biomaterial.’ A systematic literature search was performed to identify relevant articles that describes the use of PRF, through PubMed and Scopus databases. The focus of this article is to explore the role of PRF in esthetic facial surgery.
Keywords: Platelet rich fibrin, platelet aggregates, facial plastic surgery