Abstract: cN-II class of 5' purine nucleotidases exhibit specificity for
IMP/GMP and belong to the HAD (haloacid dehalogenase) superfamily of
hydrolases. The recently identified ISN1 class of IMP specific 5'-nucleotidases
occurring in yeast, fungi and certain Plasmodia lack sequence homology with the
cN-II class of enzymes. We show from analysis of motif and fold conservation
that ISN1s also belong to the HAD superfamily. This identification adds a new
novel member to this superfamily.