ISN1 Nucleotidases and HAD Superfamily Protein Fold: In silico Sequence and Structure Analysis

Article type: Research Article

Authors: Srinivasan, Bharath | Balaram, Hemalatha

Affiliations: Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore 560 064, India

Note: [] Corresponding author. Tel.: +91 80 22082812; Fax: +91 80 22082766; E-mail: [email protected]

Abstract: cN-II class of 5' purine nucleotidases exhibit specificity for IMP/GMP and belong to the HAD (haloacid dehalogenase) superfamily of hydrolases. The recently identified ISN1 class of IMP specific 5'-nucleotidases occurring in yeast, fungi and certain Plasmodia lack sequence homology with the cN-II class of enzymes. We show from analysis of motif and fold conservation that ISN1s also belong to the HAD superfamily. This identification adds a new novel member to this superfamily.

Keywords: Purine nucleotidase, ISN1 nucleotidase, HAD superfamily, distant homology modeling, phylogenetic analysis

Journal: In Silico Biology, vol. 7, no. 2, pp. 187-193, 2007