High affinity human antibodies by phage display

Article type: Research Article

Authors: Aujame, L.^{; *} | Geoffroy, F. | Sodoyer, R.

Affiliations: Department of Molecular Biology, Pasteur Mérieux Connaught, 69280 Marcy l'Etoile, France

Correspondence: [*] Correspondence and reprint requests to: L. Aujame, Department of Molecular Biology, Pasteur Mérieux Connaught, 1541 Avenue Marcel Mérieux, 69280 Marcy l'Etoile, France.

Abstract: The development of phage display has now made it possible to consider the isolation of human antibodies directly without immunization. Recent advances in the field of human immunogenetics and in phage technology have led to the assembly of ‘naive’ human repertoires in vitro whose complexity approach that of the natural immune system. Screening of these libraries has allowed the isolation in one step of antibodies with affinities in the nanomolar range.

Keywords: Human antibodies, phage display, selection, combinatorial library

DOI: 10.3233/HAB-1997-8401

Journal: Human Antibodies, vol. 8, no. 4, pp. 155-168, 1997