Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Bender, Eckharda; b | Woof, Jennifer M.a | Atkin, Julie D.a | Barker, Mike D.a | Bebbington, Chris R.c | Burton, Dennis R.a; b;
Affiliations: [a] Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK | [b] Departments of Immunology and Molecular Biology, Scripps Research Institute, La Jolla, CA, USA | [c] Celltech Ltd., Slough, UK
Note: [] Address reprint requests to Dr. Dennis R. Burton, The Scripps Research Institute, 10666 North Torrey Pines Road, La Jolla, CA 92037.
Abstract: The combinatorial phage library approach to immunoglobulin repertoire cloning recently made it possible to isolate gene fragments encoding human immunoglobulin G1 Fabs binding with high affinity to specific antigens. Here we describe the construction of genes encoding whole human anti–tetanus toxoid antibodies based on one of these gene fragments and the efficient expression of these constructs by co-transfection of separate heavy and light chain vectors into a Chinese hamster ovary cell line constitutively expressing a viral transactivator protein. This system will be generally useful for the rapid analysis of recombinant antibodies derived from combinatorial libraries.
Keywords: human antibody, immunoglobulin repertoire cloning, CHO cells, tetanus toxoid
DOI: 10.3233/HAB-1993-4205
Journal: Human Antibodies, vol. 4, no. 2, pp. 74-79, 1993
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
[email protected]
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office [email protected]
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
如果您在出版方面需要帮助或有任何建, 件至: [email protected]