Optimized protocol for soluble prokaryotic expression, purification and refolding of the human inhibin α subunit, a cysteine rich peptide chain

Article type: Research Article

Authors: Kolivand, Sedighe^a | Nazari, Mahboobeh^b | Modarressi, Mohammad Hossein^c | Najafabadi, Mohammad Reza Hosseini^d | Hemati, Atefeh^e | Ghafouri-Fard, Soudeh^{f; *} | Motevaseli, Elahe^{g; *}

Affiliations: [a] Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran | [b] Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran | [c] Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran | [d] Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran | [e] Department of Cell and Molecular Biology, School of Biological Sciences, Kharazmi University, Tehran, Iran | [f] Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran | [g] Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Correspondence: [*] Corresponding authors: Soudedeh Ghafouri-Fard, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Tel./Fax: +98 2123872572; E-mail: s.ghafourifard@ sbmu.ac.ir; Elahe Motevaseli, Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Tel./Fax: +98 2123872572; E-mail: [email protected].

Abstract: BACKGROUND: Inhibin A, a member of TGF-β superfamily, consists of α and β subunits. These subunits contain several cysteine residues in amino acid sequence that forms inter- and intra-subunits disulfide bonds. Due to the reducing environment of the bacterial cytoplasm, disulfide bonds formation in E.coli cytoplasm is not possible. Therefore, this can cause misfolding, aggregation and inclusion bodies formation during protein expression. As a result, the expression of inhibin subunits in E.coli produces inclusion bodies OBJECTIVE: We aimed at identification of an optimized protocol for expression and recovery of inhibin α-subunit from inclusion bodies. METHODS: Two vectors, four different E.coli strains, and six solubilization conditions for were used for the optimization of inhibin α-subunit production. Then, the solubilized proteins were purified through Ni-NTA affinity chromatography, characterized by SDS-PAGE and Western blotting (WB) using anti-his tag antibody, and refolded by dilution. RESULTS: The results showed that inhibin α-subunits were successfully expressed in both vectors and the pET22b+inhibin α-subunit in ShuffleTM T7 strain had the highest expression; however, most of the expression was in an insoluble form. Among solubilization buffers examined, a buffer containing 2M urea with pH 12 was the best buffer to dissolve the insoluble protein. The high purity of protein was confirmed by SDS-PAGE and WB. Non-reducing SDS-PAGE demonstrating inhibin α-subunit refolded well. CONCLUSION: The current protocol is an efficient method for protocol for expression and recovery of inhibin α-subunit from inclusion bodies.

Keywords: Inhibin α-subunit, recombinant protein, expression optimization, inclusion body solubilization, protein refolding

DOI: 10.3233/HAB-190399

Journal: Human Antibodies, vol. 28, no. 2, pp. 131-139, 2020