Developing and mature human granulocytes express ELP 6 in the cytoplasm

Article type: Research Article

Authors: Wagner, Lori A.^{a; *} | Wang, Shuping^b | Wayner, Elizabeth A.^c | Christensen, Clarissa^d | Perkins, Sherrie J.^b | Ward, Gavin W.^a | Weiss, Robert B.^e | Dunn, Diane M.^e | Redd, Michael J.^f | Spangrude, Gerald J.^g | Gleich, Gerald J.^a

Affiliations: [a] Department of Dermatology, University of Utah, Salt Lake City, UT, USA | [b] Department of Biochemistry, University of Utah, Salt Lake City, UT, USA | [c] Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment, Seattle, WA, USA | [d] BioFire Diagnostics Inc., Salt Lake City, UT, USA | [e] Eccles Institute of Human Genetics, University of Utah, Salt Lake City, UT, USA | [f] 2000 Circle of Hope Huntsman Cancer Center, Salt Lake City, UT, USA | [g] Department of Hematology, University of Utah, Salt Lake City, UT, USA

Correspondence: [*] Corresponding author: Lori A. Wagner, Department of Dermatology, University of Utah, 30 North, 1900 East, Salt Lake City, UT 84132, USA. Tel.: +1 801 230 7290; E-mail: [email protected].

Abstract: Background:c3orf75 is a conserved open reading frame within the human genome and has recently been identified as the Elongator subunit, ELP6 [1]. The Elongator enzyme complex has diverse roles, including translational control, neuronal development, cell migration and tumorigenicity [2]. Objective:To identify genes expressed early in human eosinophil development. Methods:Eosinophilopoiesis was investigated by gene profiling of IL-5 stimulated CD34+ cells; ELP6 mRNA is upregulated. A monoclonal antibody was raised to the recombinant protein predicted by the open reading frame. Results:ELP6 transcripts are upregulated in a human tissue culture model of eosinophil development during gene profiling experiments. Transcripts are expressed in most tissue types, as shown by reverse-transcriptase PCR. Western blot experiments show that human ELP6 is a 30 kDa protein expressed in the bone marrow, as well as in many other tissues. Flow cytometry experiments of human bone marrow mononuclear cells show that ELP6 is expressed intracellularly, in developing and mature human neutrophils, eosinophils and monocytes. Conclusions:ELP6 is expressed intracellularly in developing and mature granulocytes and monocytes but not in lymphocytes and erythrocytes.

Keywords: Granulopoiesis, ELP6, bone marrow, antibody, human

DOI: 10.3233/HAB-130268

Journal: Human Antibodies, vol. 22, no. 1-2, pp. 21-29, 2013