Generation of mouse-human hybridomas secreting human monoclonal antibodies to Japanese cedar pollen allergen Cry j1

Article type: Research Article

Authors: Naganawa, Yasunori^a | Shinmoto, Hiroshi^{a; *} | Shimmoto, Michie^b

Affiliations: [a] National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki, 305-8642, Japan | [b] The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Hon-komagome, Bunkyo-ku, Tokyo, 113-8613, Japan

Correspondence: [*] Corresponding author. Tel./Fax: +81 29 838 8055; E-mail: [email protected]

Abstract: Peripheral blood lymphocytes from 13 donors were transformed with Epstein-Barr virus (EBV) to establish immortalized human B-cells secreting antibodies to Japanese cedar (Cryptomeria japonica) pollen allergens. EBV-transformed B-lymphocytes were then fused with mouse myeloma SP2/O3, and three clones of mouse-human hybridomas secreting human IgM class monoclonal antibodies to a cedar pollen allergen Cry j1 were established.

Keywords: Human-mouse hybridoma, cedar pollen allergen, human monoclonal antibody, epitope, IgM, Epstein-Barr virus

DOI: 10.3233/HAB-2005-141-205

Journal: Human Antibodies, vol. 14, no. 1-2, pp. 27-31, 2005