Phage antibodies from combinatorial library neutralize vaccinia virus

Article type: Research Article

Authors: Tikunova, N.V. | Morozova, V.V. | Batanova, T.A. | Belanov, E.F. | Bormotov, N.I. | Ilyichev, A.A.

Affiliations: Research Center of Virology and Biotechnology “Vector”, Koltsovo, Novosibirsk region, 630559, Russia. Tel.: +7 3832 366 570; Fax: +8 3832 367 409; E-mail: [email protected]

Abstract: The library of human scFv antibodies displayed on the surface of bacteriophages was panned against Vaccinia virus (VACV), strain Elstree. 75% binding with Vaccinia virus. 5 clones were characterized for their binding with VACV and their ability to neutralize VACV in plaque reduction neutralization test (PRNT). Antibodies from the clones were obtained as soluble individual molecules and their binding activities were confirmed in ELISA.

DOI: 10.3233/HAB-2001-103-401

Journal: Human Antibodies, vol. 10, no. 3-4, pp. 95-99, 2001