Affiliations: Molecular Oncology Laboratory, Medical School, University of Patras, Rion, Greece | Department of Anatomy–Histology–Embryology, Medical School, University of Patras, Rion, Greece | Department of Surgery, Medical School, University of Patras, Rion, Greece | Department of Pathology, Medical School, University of Patras, Rion, Greece
Note: [] Corresponding author: Haralabos P. Kalofonos, Clinical Oncology Laboratory, Division of Oncology, Department of Medicine, Medical School, University of Patras, 265 04 Rion, Patras, Greece. Tel.: +30 2610 999535; Fax: +30 2610 994645; E-mail: [email protected].
Abstract: Background: Survivin is involved in the regulation of cell division and survival, two key processes in cancer. The majority of studies on survivin in colorectal cancer (CRC) have focused on protein expression and less is known about the expression of survivin splicing variants or survivin gene polymorphisms in CRC. In the present study, the mRNA levels of the five known isoforms of survivin as well as survivin protein were assessed in matched normal and neoplastic colorectal tissue. Moreover, the 9386C/T and −31G/C polymorphisms were investigated. Methods: Quantitative RT-PCR was used to assess mRNA levels in fresh/frozen tissue samples. Protein levels were immunohistochemically evaluated on formalin-fixed paraffin-embedded tissue sections. Individuals were genotyped using real time PCR. Results: Expression of all 5 survivin splice variants as well as survivin protein was elevated in colorectal carcinomas compared to normal tissue. Specific splice variant expression differentially correlated with clinicopathological parameters. Furthermore, both snps correlated with splice variant levels or their ratios in colorectal carcinomas while the −31G/C snp may be related to CRC development and improved overall survival. Conclusion: Our results support a role of survivin in colorectal carcinogenesis while the −31G/C snp may constitute a marker of survival.
