High resolution comparative genomic hybridization detects 7–8 megabasepair deletion in PCR amplified DNA

Article type: Research Article

Authors: Larsen, Jacob^; | Ottesen, Anne Marie | Kirchhoff, Maria | Lundsteen, Claes | Larsen, Jørgen K.

Affiliations: Finsen Laboratory, Finsen Center, Rigshospitalet, Strandboulevarden 49, DK‐2100, Copenhagen, Denmark | Department of Growth and Reproduction, Juliane Marie Center, Rigshospitalet, Blegdamsvej 9, DK‐2100, Copenhagen, Denmark | Department of Clinical Genetics, Juliane Marie Center, Rigshospitalet, Blegdamsvej 9, DK‐2100, Copenhagen, Denmark

Note: [] Corresponding author: Jacob Larsen, Finsen Laboratory, Finsen Center, Rigshospitalet, Strandboulevarden 49, DK‐2100, Copenhagen, Denmark. Tel.: +45 3545 5751; Fax: +45 3538 5450; E‐mail: [email protected].

Abstract: We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP‐PCR) as opposed to the use of unamplified DNA. Five DNA samples from B‐cell leukemias with small 11q deletions were amplified by DOP‐PCR and analysed by means of high resolution comparative genomic hybridization (HR‐CGH) for the evaluation of aberration size detection limit. By means of HR‐CGH, we found the detection limit of DOP‐PCR CGH for deletions to be between 3 Mbp and 7–8 Mbp.

Keywords: High resolution comparative genomic hybridization, DOP‐PCR, B‐cell leukemia

Journal: Analytical Cellular Pathology, vol. 23, no. 2, pp. 61-64, 2001