Affiliations: Malaysia Genome Institute, UKM-MTDC Smart Technology
Centre, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor DE,
Malaysia | Molecular Genetics Laboratory, Faculty of Medicine and
Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE,
Malaysia | School of Biosciences and Biotechnology, Faculty of
Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi,
Selangor DE, Malaysia | Department of Medical Microbiology and Parasitology,
Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM
Serdang, Selangor DE, Malaysia
Abstract: Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse
roles in the cellular biology of many organisms, including signal transduction,
secretion and vesicular trafficking, and regulation of cytoskeleton assembly.
Discovery of the PIP5K gene in Eimeria tenella may shed light on its role
in the biology of this avian protozoan, and afford further understanding of the
cell-host interaction, particularly during the invasion process. In this study,
we report the identification of the PIP5K coding region in the genome sequence
of Eimeria tenella using in silico gene prediction approaches.
Prediction of the PIP5K coding sequence was confirmed by mapping the
full-length cDNA sequence, generated via the Rapid Amplification of cDNA Ends
(RACE) method, to the genomic sequence. The putative PIP5K gene of Eimeria
tenella is located on the complementary strand of the E1080B12.b1 contig, and
comprises 12 exons. Further analysis showed that the coding region spans from
exon 1 to exon 7, with all exons obeying the adopted 'gt
... ag' splicing rule of intronic sequences. Consensus of
the hexameric 5' donor-splice site was deduced as GTRDBB
... and the consensus for the 3' acceptor-splice sites as
...BHDYAG. The gene encodes a 252-amino acid residue protein. Domain search and
protein fold recognition analyses provide compelling evidences that the deduced
protein is a PIP5K.
