Affiliations: Department of Biophysics, Marmara University School of Medicine, Istanbul, Turkey | Biological Sciences and Bioengineering, Sabanci University, Orhanli, Tuzla, Istanbul, Turkey | Department of Biology, Middle East Technical University, Ankara, Turkey | Department of Electrical and Electronics Engineering, Middle East Technical University, Ankara, Turkey | Laboratory of Enzymology and Protein Folding, Centre for Protein Engineering, Institut de Chimie B6, University of Liège, Liège, Belgium | Department of Biophysics, School of Medicine, Acibadem University, Istanbul, Turkey
Note: [] These authors contributed equally to this work.
Abstract: Go, a member of heterotrimeric guanine nucleotide-binding proteins, is the most abundant form of G protein in the central and peripheral nervous systems. Goα has a significant role in neuronal development and function but its signal transduction mechanism remains to be clarified. In this study, the bovine Goα subunit was overexpressed and purified into homogeneity. Its activity was studied using [35S] GTPγS binding, intrinsic fluorescence and BODIPY assays. The secondary structure was determined by both FTIR and CD spectroscopy as 42.3% α-helix, 13.4% β-sheet and 24.3% β-turn. A theoretical structure model was constructed. The structure from homology modeling is in very good agreement with the crystal structure of mouse Goα subunit except for the loop between αB–αC helices. This model was docked to the mouse RGS16 molecule. T117 on the αB–αC loop of Goα interacted with K172 on RGS16 as opposed to the T117 and K164 interaction in mouse.
