A validated direct spectrofluorimetric method for quantification of mirtazapine in human whole blood

Article type: Research Article

Authors: Saini, Balraj | Kaushal, Manjula | Bansal, Gulshan

Affiliations: Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India

Note: [] Corresponding author: Gulshan Bansal, Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, 147001 India. Tel.: +91 175 3046255; Fax: +91 175 2283073; E-mail: [email protected].

Abstract: A spectrofluorimetric method for estimation of mirtazapine in human whole blood was developed and validated. The recovery efficiency of the processing method was 95–98%. The analytical method was linear over drug concentration of 10–200 ng/ml. The limit of quantification was 10 ng/ml. The method was precise with %RSD for intra-day and inter-day precision being <3.0 and 1.5, respectively. Excellent recoveries (97.87–99.69%) were achieved during accuracy studies. The method was robust to small changes in processing method and instrumental parameters. The present method can be employed for direct fluorimetric determination of mirtazapine in human whole blood during clinical studies.

Keywords: Mirtazapine, spectrofluorimetry, protein precipitation, human blood, validated

DOI: 10.3233/SPE-2010-0488

Journal: Spectroscopy, vol. 24, no. 6, pp. 641-649, 2010