FTIR spectroscopy for detection and identification of fungal phytopathogenes
Issue title: From Molecule to Tissue: XIII European Conference on the Spectroscopy of Biological Molecules, Palermo, Italy, August 28–September 2, 2009, Part 2 of 2
Affiliations: Department of Physics, SCE-Sami Shamoon College of Engineering, Beer-Sheva, Israel | Department of Plant Pathology, The Institute of Plant Protection, Agricultural Research Organization, Gilat Experiment Station, Bet Dagan, Israel | Department of Physics, Ben-Gurion University of the Negev, Beer-Sheva, Israel | Department of Virology and Developmental Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
Note: [] Corresponding authors: M. Huleihel, Department of Virology and Developmental Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel. Tel.: +972 8 6479867; Fax: +972 8 6479867; E-mail: [email protected]. S. Mordechai, Department of Physics, Ben-Gurion University, Beer-Sheva 84105, Israel. Tel.: +972 8 6461749; Fax: 972 8 6472903; E-mail: [email protected].
Abstract: Soil-borne fungi are considered as major pathogens to many plants and can cause a severe economic damage. Early detection and identification of these pathogens is very important and might be critical for their control. The available methods for identification of fungi like molecular biology, serological tests and PCRs tests (polymerase chain reaction) are time consuming and not always very specific. Fourier-transform infrared (FTIR) attenuated total reflection (ATR) spectroscopy, is considered to be a comprehensive and sensitive method for detection of molecular changes in intact cells. In the present study we used FTIR–ATR as a sensitive and effective assay for the detection and discrimination between different fungal genera. Our results showed significant spectral differences between the various examined fungi genera. These results proved the possibility of discrimination between these fungi on the genus level.
