Towards instantaneous quantitative fluoroimaging drugs determination in body fluids with no added reagents

Issue title: From Molecule to Tissue: XIII European Conference on the Spectroscopy of Biological Molecules, Palermo, Italy, August 28–September 2, 2009, Part 2 of 2

Article type: Research Article

Authors: Strashnikova, Natalia V. | Gershanik, Arcady P. | Papiashvili, Nona | Khankin, Daniel | Cohen-Luria, Rivka | Mark, Shlomo | Kalisky, Yehoshua | Parola, Abraham H.^;

Affiliations: Department of Chemistry, Ben Gurion University, Beer Sheva, Israel | Department of Software Engineering, Shamoon College of Engineering, Beer Sheva, Israel | Chemistry Department, Nuclear Research Center Negev, Beer Sheva, Israel

Note: [] Corresponding author: Prof. Abraham H. Parola, Department of Chemistry, Ben Gurion University, P.O. Box 653, Beer Sheva, 84105, Israel. Fax: +972 8 647 2943; E-mail: [email protected].

Abstract: Our objective is to develop a simple monitoring technique for rapid, sensitive and quantitative drugs detection in body fluids, with no reagent added and no need for qualified professionals. The user-friendly automatic Fluo-imager will (a) measure the full-range 3D fluorescence map of the inserted fluid sample, (b) determine the chemical nature and concentration of the drugs and (c) transfer the results through internet to the diagnosis center. For these goals the fluorescence measurement data will be examined by neuronal network-pattern recognition software. The software identifies the chemical nature and the appropriate concentration of the drug by comparison of the obtained 3D pattern with the contents of the data bank. One of the problems in the approach under consideration is the high optical density of body fluids in the UV region, which raises difficulties in the fluorescence measurements. In this paper, we have attempted to overcome this problem by means of preliminary dilution. Nevertheless, the problem of subtraction of the fluid fluorescence background still needs to be addressed.

Keywords: Therapy, drug, monitoring, Fluo-imager, 3D fluorescence, fluorescence spectra, pattern recognition software, data bank, diluted human plasma, concentration–calibration curves

DOI: 10.3233/SPE-2010-0438

Journal: Spectroscopy, vol. 24, no. 3-4, pp. 317-324, 2010