Rapid assay of picogram level of sudan I in hot chilli sauce by flow injection chemiluminescence

Article type: Research Article

Authors: Gao, Xiaofei | Liu, Houyong | Song, Zhenghua | He, Xili | Dong, Faxin

Affiliations: Department of Chemistry, Northwest University, 710069, Xi'an, China

Note: [] Corresponding author. Fax: +86 29 88303798; E-mail: [email protected].

Abstract: A novel chemiluminescence method for the assay of sudan I was designed using flow injection with chemiluminescence detection. The proposed method was based on the increment effect of sudan I on the chemiluminescence intensity in the luminol–KIO4 system. The increment of chemiluminescence intensity was correlated with the sudan I concentration in the range from 0.1 to 10 pg ml−1, and the determination could be performed in 0.5 min in flow rate of 2 ml min−1, including sampling and washing, giving a throughput of 120 h−1 with a relative standard deviation of less than 5.0%. The method had been successfully applied to the assay of sudan I in Pixian douban, Golden mark guilin chilli sauce and Golden mark satay sauce, and the recovery was 90.0–103.8%.

Keywords: Sudan I, chemiluminescence, luminol, flow injection

Journal: Spectroscopy, vol. 21, no. 2, pp. 135-141, 2007