Binding studies of paeonolum with bovine serum albumin using spectroscopic methods

Article type: Research Article

Authors: Chen, Chang-yun | Gu, Xiao-tian | Zhou, Jia-hong^;

Affiliations: Department of Chemistry, Nanjing Xiaozhuang University, Nanjing 210017, PR China | Analysis & Detecting Center, Nanjing Normal University, Nanjing 210097, PR China

Note: [] Corresponding author: Jiahong Zhou, Associate Professor, Analysis & Detecting Center, Nanjing Normal University, 122 Ninghai Road, Nanjing, Jiangsu 210097, P.R. China. Fax: +86 25 8359 8359; E-mail: [email protected].

Abstract: Fluorescence and UV spectroscopic techniques were used to investigate the interaction of Paeonolum, one of the major bioactive components isolated from the bark of peony plant, with bovine serum albumin (BSA). Results obtained reveal that a binding interaction occurs between paeonolum and BSA under physiological conditions, and the interaction can quench the fluorescence of BSA originating from the complex formation between paeonolum and BSA. In addition, according to the thermodynamics parameters of this interaction process, it appears that this binding interaction is mainly hydrophobic in nature.

Keywords: Paeonolum, bovine serum albumin, fluorescence quenching, UV absorption spectroscopy

Journal: Spectroscopy, vol. 21, no. 1, pp. 53-60, 2007