Raman spectroscopic study of photosensitive damage to lysozyme structure sensitized by hypocrellin A

Article type: Research Article

Authors: Zhou, J.H.^; | Wu, X.H. | Wei, S.H. | Gu, X.T. | Feng, Y.Y. | Wang, X.S. | Zhang, B.W.

Affiliations: Analysis & Testing Center, Nanjing Normal University, 210097 Nanjing, P.R. China | Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100101, P.R. China

Note: [] Corresponding author: Jiahong Zhou, Associate Professor, Analysis & Detecting Center, Nanjing Normal University, 122 Ninghai Road, Nanjing, Jiangsu 210097, P.R. China. Fax: +86 25 8359 8359; E-mail: [email protected].

Abstract: Laser Raman spectroscopy was used to detect the nature of the structural change in lysozyme sensitized by hypocrellin A (HA) at the molecular level. The results indicated that the orderly structure of lysozyme has been damaged by the active oxygen (1O2, O2−· and $\bfcdot$OH, etc.) generated by HA, and cause the changes in H-bonds system of the main chain and the side chain of lysozyme.

Keywords: Hypocrellin A, lysozyme, photosensitive damage, Raman spectroscopy, protein conformation

Journal: Spectroscopy, vol. 20, no. 5-6, pp. 269-273, 2006