Protein characterization using Liquid Chromatography Desorption Ionization on Silicon Mass Spectrometry (LC‐DIOS‐MS)

Article type: Research Article

Authors: Prenni, Jessica E. | Shen, Zhouxin | Trauger, Sunia | Chen, Wei | Siuzdak, Gary^;

Affiliations: The Scripps Research Institute, Center for Mass Spectrometry, Department of Molecular Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA | Mass Consortium Corporation, 3030 Bunker Hill Street, Suite 104, San Diego, CA 92109, USA

Note: [] Corresponding author. Tel.: +1 858 748 9415; Fax: +1 858 784 9496; E‐mail: [email protected].

Abstract: This paper presents a novel combination of off‐line liquid chromatography (LC) separation with desorption ionization on silicon mass spectrometry (DIOS‐MS) as an alternative to the traditional on‐line coupling of LC and electrospray ionization (ESI). In this work, electrospray deposition is used to generate a spatially preserved linear track of the separated sample on a specially designed DIOS chip. The total sequence coverage analysis of two model protein systems was evaluated by both LC‐DIOS‐MS and nanoLC ESI tandem MS (MS/MS). LC‐DIOS‐MS yielded improved sequence coverage for both of the model systems (between 99.5 and 100%) compared with traditional LC‐ESI‐MS/MS analysis (between 82 and 87.6%). In addition to improved sequence coverage determination, LC‐DIOS‐MS also offers the potential for high‐throughput protein characterization.

Journal: Spectroscopy, vol. 17, no. 4, pp. 693-698, 2003