Biosensors based on nucleic acid interaction

Issue title: First International Conference on Biomedical Spectroscopy: From molecules to men, Cardiff, UK, 7–10 July 2002, Part II

Article type: Research Article

Authors: Minunni, M.

Affiliations: Biosensor Laboratory, Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy Tel.: +39 055 4573314; Fax: +39 055 4573384; E‐mail: [email protected]

Abstract: DNA sensing is an emerging technology based on hybridisation reaction between an immobilised DNA probe and a molecular target, consisting of a probe complementary sequence in solution. Many application have been developed in the field of environmental, food and clinical analysis. Surface plasmon resonance and piezoelectric sensing are reported as transduction principles for DNA‐based devices. These techniques are able to monitor in real‐time and without the use of any label the hybridisation reaction between nucleic acids. Particular attention is given to Genetically Modified Organism detection.

Keywords: Biosensor, DNA sensing, nucleic acids, hybridisation reaction

Journal: Spectroscopy, vol. 17, no. 2-3, pp. 613-625, 2003