Magnetization‐transfer ³¹P NMR of biochemical exchange in vivo: Application to creatine kinase kinetics

Article type: Research Article

Authors: Möller, Harald E.^; | Wiedermann, Dirk

Affiliations: Max‐Planck‐Institut für neuropsychologische Forschung, Stephanstraße 1a, D‐04103 Leipzig, Germany | Max‐Planck‐Institut für neurologische Forschung, Gleueler Straße 50, D‐50866 Köln, Germany

Note: [] Corresponding author. Tel.: +49 341 9940 212; Fax: +49 341 9940 221; E‐mail: [email protected].

Abstract: Phosphorus‐31 saturation‐transfer NMR spectroscopy provides an elegant means to study fluxes through the creatine kinase reaction in human skeletal muscle. To obtain reliable quantitative kinetic information, experimental imperfections, such as incomplete saturation and radiofrequency bleed over need to be addressed appropriately. In resting muscle, creatine kinase was near equilibrium both in normal controls and in a patient with impaired oxidative phosphorylation. Oral intake of high doses of creatine monohydrate for several days resulted in significantly increased concentrations of phosphocreatine but had no measurable effect on the phosphocreatine resynthesis rate in resting muscle.

Journal: Spectroscopy, vol. 16, no. 3-4, pp. 207-216, 2002