Temperature induced conformational transitions of elastin‐like polypentapeptides studied by Raman and NMR spectroscopy

Article type: Research Article

Authors: Dybal, Jiří^; | Schmidt, Pavel | Kurková, Dana | Kříž, Jaroslav | Rodríguez‐Cabello, José Carlos | Alonso, Matilde

Affiliations: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06 Prague 6, Czech Republic | Department of Condensed Matter, E.T.S.I.I., University of Valladolid, 47011 Valladolid, Spain | Department of Analytical Chemistry, E.U.P., University of Valladolid, 47014 Valladolid, Spain

Note: [] Corresponding author. Tel.: +420 2 20403387; Fax: +420 2 367981; E‐mail: [email protected].

Abstract: Raman and NMR spectroscopy together with DFT quantum chemical calculations have been used to elucidate conformational changes occurring during inverse temperature transition in water solution of two elastin‐like polypentapeptides, poly(G1V1G2V2P) and poly(AV1GV2P). From the temperature dependences of Raman spectra and 1H–1H NOESY spectra follows that above the transition temperature a β(II)‐turn conformational structure is formed on the residue located between Pro and Val1.

Journal: Spectroscopy, vol. 16, no. 3-4, pp. 251-255, 2002