Affiliations: [a] Uludag University Medical School Department of Physiology, Bursa, Turkey | [b] Uludag University Medical School Department of Pharmacology, Bursa, Turkey | [c] Kanuni Sultan Suleyman Training and Research Hospital, Department of Pediatrics, Division of Neonatology, Istanbul, Turkey
Correspondence:
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Correspondence to: Prof. Tulin Alkan, M.D., Ph.D., Uludag University Medical School, Department of Physiology, Bursa 16059, Turkey. Tel.: +90 224 295 4016; Fax: +90 224 442 8855; [email protected]
Abstract: Purpose: A significant cause of neurological disability in newborns is hypoxic-ischemic encephalopathy (HIE), a disorder which involves an enhancement in histone deacetylase (HDAC) activity among underlying pathological mechanisms. We showed recently that exogenous administration of uridine to newborn rats with HIE reduced brain injury in a dose-dependent manner. The present study was performed to investigate whether uridine modulates histone acetylation/deacetylation balance in a neonatal rat model of HIE. Methods: Newborn rats that were subjected to hypoxic-ischemic (HI) insult on postnatal day 7 (P7) were injected intraperitoneally with either saline or uridine (500 mg/kg) for three consecutive days. One day after completion of treatment, brains of pups were collected for evaluation of brain infarct volume, apoptosis, HDAC activity and acetylated-Histone H3 (Ac-H3) and H4 (Ac-H4) protein levels. Results: Results revealed that uridine administration reduced infarct volume, active Caspase-3 levels and HDAC activity while increasing the expressions of Ac-H3 and Ac-H4 proteins. Conclusions: We conclude that one mechanism by which uridine provides neuroprotection in neonatal rat HIE model involves reduction in HDAC activity.
Keywords: Hypoxic-ischemic encephalopathy, neonatal, rat, uridine, histone deacetylase, neuroprotection
