Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Meredith, Ruby F.a; | Khazaeli, M.B.a | Grizzle, William E.a | Orr, Roger A.a | Plott, Genea | Urist, Marshall M.a | Liu, Tiepua | Russell, Charles D.a | Wheeler, Richard H.a | Schlom, Jeffreyb | LoBuglio, Albert F.a
Affiliations: [a] University of Alabama at Birmingham Comprehensive Cancer Center | [b] Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, MD, USA
Note: [] Address reprint requests to Dr. Ruby F. Meredith, Department of Radiation Oncology, University of Alabama at Birmingham Comprehensive Cancer Center, L. B. Wallace Tumor Institute 117, 1824 Sixth Avenue South, Birmingham, AL 35294-3300, USA.
Abstract: To compare radiolocalization of murine B72.3 (m-B72.3) and mouse/human chimeric B72.3 (ch-B72.3) antibodies, five patients with biopsy confirmed adenocarcinoma of the colon received both radio labeled antibodies 4 or 7 days before laparotomy. Following antibody administration, preoperative gamma camera images showed localization to sites of disease in four of the five patients. Autoradiography of resected specimens showed that both labeled antibodies localized specifically to the tumor with only minimal amounts in normal tissues. Radioactivity from each isotope in biopsy specimens of tumor and normal tissues was quantitated by scintillation gamma counting. Comparison of the percentages of injected activities for each antibody in resected tumor and normal tissue yields tumor to normal tissue radiolocalization ratios of 2.7–13.3 and 0.9–6.3 for murine and chimeric antibodies, respectively. The higher ratios for murine antibody were due to lower normal tissue levels, reflecting its faster clearance from the circulation, whereas the quantitative uptake of labeled antibody was always greater with the chimeric antibody. The chimera to murine antibody ratios in tumor of 1.1–2.7 suggest modest enhancement of tumor localization with chimeric antibody because of its longer half-life.
Keywords: antibody, chimera, radiolabel, localization, quantitation
DOI: 10.3233/HAB-1993-4406
Journal: Human Antibodies, vol. 4, no. 4, pp. 190-197, 1993
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
[email protected]
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office [email protected]
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
如果您在出版方面需要帮助或有任何建, 件至: [email protected]