Affiliations: Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA | Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA | McGowan Institute of Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA | Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, USA
Note: [] Address for correspondence: Zhongjun J. Wu, PhD, Department of Surgery, University of Maryland School of Medicine, MSTF 434F, 10 South Pine Street, Baltimore, MD 21201, USA. Tel.: +1 410 706 7715; Fax: +1 410 706 0311; E-mail: [email protected].
Abstract: The understanding of erythrocyte deformation under conditions of high shear stress and short exposure time is central to the study of hemorheology and hemolysis within prosthetic blood contacting devices. A combined computational and experimental microscopic study was conducted to investigate the erythrocyte deformation and its relation to transient stress fields. A microfluidic channel system with small channels fabricated using polydimethylsiloxane on the order of 100 μm was designed to generate transient stress fields through which the erythrocytes were forced to flow. The shear stress fields were analyzed by three-dimensional computational fluid dynamics. Microscopic images of deforming erythrocytes were experimentally recorded to obtain the changes in cell morphology over a wide range of fluid dynamic stresses. The erythrocyte elongation index (EI) increased from 0 to 0.54 with increasing shear stress up to 123 Pa. In this shear stress range, erythrocytes behaved like fluid droplets, and deformed and flowed following the surrounding fluid. Cells exposed to shear stress beyond 123 Pa (up to 5170 Pa) did not exhibit additional elongation beyond EI=0.54. Two-stage deformation of erythrocytes in response to shear stress was observed: an initial linear elongation with increasing shear stress and a plateau beyond a critical shear stress.
Keywords: Red blood cell, blood damage, shear stress, elongation index
Journal: Biorheology, vol. 43, no. 6, pp. 747-765, 2006
