Affiliations: Laboratorio di Immunologia e Genetica, Istituto di Ricerca Codivilla Putti, Istituti Ortopedici Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy | Dipartimento di Medicina Interna e Gastroenterologia, Università di Bologna, Via Massarenti 9, 40138 Bologna, Italy
Note: [] Address for correspondence: Brunella Grigolo, PhD, Laboratorio di Immunologia e Genetica, Istituto di Ricerca Codivilla Putti, I.O.R., Via di Barbiano 1/10, 40136 Bologna, Italy. Tel.: +39 051 6366803/6366802; Fax: +39 051 6366807; E-mail: [email protected].
Abstract: The development of improved methods for treatment of chondral defects using autologous cells in combination with biomaterials leads to a new generation of implantable devices. Their association gives rise to a hybrid construct combining biological and material components that can be specifically committed. The comprehension of cellular and molecular mechanisms of cartilage repair and the use of biomaterials in combination with chondrocytes or mesenchymal stem cells in the treatment of cartilage defects has opened a new era of therapeutical strategies. Recently, their applicability in the treatment of early lesions in osteoarthritis is under investigation. To obtain new information on the behaviour of chondrocytes and mesenchymal stem cells grown on a hyaluronan derivative scaffold (Hyaff®-11) already used in cartilage repair, we analysed a series of molecules expressed by these cells by Real-Time RT-PCR and immunohistochemical analyses. The data obtained with this work showed that this biomaterial is able to reduce the expression of some catabolic molecules by human chondrocytes and provide a good environment to support the differentiation of mesenchymal stem cells in chondrogenic sense. These observations confirm Hyaff®-11 as a suitable scaffold both for chondrocytes and mesenchymal stem cells for the treatment of articular cartilage defects.
Keywords: Human chondrocytes, mesenchymal stem cells, hyaluronan-based scaffold, chondrogenic differentiation
Journal: Biorheology, vol. 43, no. 3-4, pp. 471-480, 2006
