Affiliations: Cell and Developmental Biology Department, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil
Note: [] Address for correspondence: M.F. Santos, Cell and Developmental Biology Department, Av. Prof. Lineu Prestes 1524 sala 434, São Paulo, SP, Brazil, CEP 05508-900.
Abstract: We have previously shown that a mandibular propulsive appliance (MPA) stimulates cell proliferation and the synthesis of growth factors in the rat condylar cartilage. The aim of this study was to evaluate the effects of a MPA in the distribution of the integrin subunits α1 and α2 in this cartilage. Twenty eight days-old male Wistar rats were divided into treated (T) and age-matched control groups (C). Treated rats wore the appliance during 3, 5, 7, 9, 11, 15, 20, 30 and 35 days. The condyles were fixed, decalcified and paraffin-embedded. The distribution of α1 and α2 was studied by immunohistochemistry. Alpha1 distribution was uniform along the cartilage, increasing in 48 days-old rats (C20). Treated animals anticipated this increase to the age of 36 days (T9). The number of α2-positive cells was increased in C9 in the anterior condylar region, in C9 and C20 in the middle region and showed no differences in the posterior region. The MPA apparently abolished all variations, leading to a single increase at T30 in all regions. These results suggest that integrins containing the α1 and α2 subunits are modulated by forces promoted by the MPA, participating of the biological response to this therapy.
