Affiliations: Department of Biological Engineering, University of Missouri-Columbia, Columbia, MO, USA
Note: [] Address for correspondence: Mark A. Haidekker, Department of Biological Engineering, University of Missouri-Columbia, 252 Ag Engineering Building, Columbia, MO 65211, USA. Tel.: +1 573 884 4943; Fax: +1 573 882 1115; E-mail: [email protected].
Abstract: Many disease states have associated blood viscosity changes. Molecular rotors, fluorescent molecules with viscosity sensitive quantum yields, have recently been investigated as a new method for biofluid viscosity measurement. Current viscometer measurements are complicated by proteins adhering to surfaces and forming air-surface layers. It is unknown at this time what effects proteins may have on biofluid viscosity measurements using molecular rotors. To answer this question, binding affinities to blood plasma proteins were investigated by equilibrium dialysis for four hydrophilic molecular rotors. Aqueous solutions of 9-[(2-cyano-2-hydroxy-carbonyl)vinyl]julolidine (CCVJ) and three derivatives were prepared and dialyzed against solutions of bovine source albumin, fibrinogen and immunoglobulin G approximating normal physiologic concentrations and fresh-frozen human plasma. After equilibration, dye concentration on each side of the dialysis membrane was assessed by spectrophotometry. The relative binding affinity of the four dyes to the proteins and to the plasma was compared. Affinity of all dyes was highest for albumin. The bound dye fraction showed little change in relation to protein concentration in the physiological concentration range. Diol, the most hydrophilic molecular rotor tested showed the lowest affinity for albumin. This study indicates that hydrophilic molecular rotors are well-suited for biofluid viscosity measurement.
Keywords: Viscosity, TICT, CCVJ
Journal: Biorheology, vol. 42, no. 5, pp. 335-344, 2005
