Affiliations: Department of Physiology and Biophysics, Keck School of Medicine, Los Angeles, CA, USA | Algorhythms LLC, Doylestown, PA, USA
Note: [] Address for correspondence: Dr. Herbert J. Meiselman, Dept. of Physiology and Biophysics, Keck School of Medicine, University of Southern California, 1333 San Pablo Street, Los Angeles, CA 90033, USA. Tel.: +1 323 442 1268; Fax: +1 323 442 1617; E‐mail: [email protected].
Abstract: The technical complexity of previous rheometers has tended to limit the availability of blood viscosity data obtained over a wide range of shear rates. However, an automated tube‐type viscometer, the Rheolog™, has been developed; it employs a disposable flow assembly and less than five minutes are required to obtain blood viscosity results over a shear rate range of 1–1500 s−1. We have carried out validation studies of the Rheolog™ using normal human blood and have compared these results with those obtained by cone‐plate and Couette viscometers; storage time and temperature effects were also evaluated. Replicate measurements indicated mean CV levels less than 5%, and were independent of hematocrit and shear rate. Rheolog™ blood viscosity data agreed closely with those from other viscometers: average Rheolog™ differences from mean cone‐plate and Couette values were −0.3% at 28% hematocrit, −1.4% at 41% hematocrit (i.e., native), and 1.0% at 56% hematocrit. Storage at room temperature up to 8 hours and at 4°C up to 4 days had minimal effects whereas notable changes were observed when stored for 3 hours at 37°C. Our results indicate that, within the hematocrit and shear rate limits employed herein, the Rheolog™ provides rapid, accurate and reproducible blood viscosity data, and suggest its usefulness for both basic science and clinical studies.
