Adsorption of bovine serum albumin onto glassy carbon in a couette flow. Effect of shear rate on the adsorption kinetics and on the structure of the adsorbed proteic layer

Article type: Research Article

Authors: Lacour, F. | de Ficquelmont-Loizos, M. Marie | Caprani, A.

Affiliations: CNRS URA 343 “Biorheologie et Hydrodynamique Physico-Chimique” Universite Paris VII, 2 place Jussieu - 75251 PARIS Cedex 05

Note: [] Accepted by: Editor D. Quemada

Abstract: Adsorption of bovine serum albumin onto glassy carbon is investigated by analysing the time-variation of the double-layer capacitance recorded during the adsorption process. The effect of shear rate is investigated under laminar conditions in a Couette flow. Stationary and sinusoidally modulated values of the shear rate are imposed over the (0–200 s−1) range. The flow conditions are shown to play an essential role by markedly modifying the rate of all the steps (three at most) involved in the adsorption mechanism. Moreover, the structure of the adsorbed layers in the intermediate and final states are also strongly modified, an increase of the shear rate increasing the interaction between the protein and the electrode. Piezoelectric properties of albumin are invoked to account for the experimental results.

Keywords: Bovine Serum Albumin, Glassy Carbon, Couette Flow, Shear Rate, Adsorption, Kinetics, Piezoelectricity

DOI: 10.3233/BIR-1992-292-307

Journal: Biorheology, vol. 29, no. 2-3, pp. 273-284, 1992