A new automated method of image analysis: Comparison of ciliary and flagellar beats

Issue title: Proceedings of the Seventh International Congress of Biorheology. Part I. Palais des Congrès, Nancy, France, 18–23 June 1989. Dedicated to Richard Skalak

Guest editors: Alfred L. Copley and Jean-François Stoltz

Article type: Research Article

Authors: Schoevaert, D.^a | Marano, F.^b | Serres, C.^a | Berrebah, H.^b

Affiliations: [a] Laboratoire d’Histologie Embryologie Cytogénétique, CHU Bicètre 78 rue du general Leclerc 94270 Le Kremlin Bicetre, France | [b] Laboratoire de Cytophysiologie et de toxicologie cellulaire, UA CNRS 567, Université Paris VII, 2 place Jussieu 75251 Paris Cedex 05, France

Note: [] Accepted by: Editor E. Puchelle

Abstract: A new automated method of image analysis of sperm flagellar (human) and cilia (Dunaliella) bends is developed. This method permits an automatic determination of the line characterizing the flagellum. Two dynamic parameters are measured: the wave propagation velocity and the wave curvature radius. The data reveal similar patterns in the propagation of the principal and reverse waves between flagelated and ciliated cells. Conversely, differences are seen in principal wave curvature due perhaps to the presence of periaxonemal structures in the flagellum, absent in cilium. The identical patterns of reverse wave curvature in both systems may be linked to axonemal limitations.

Keywords: Image analysis, modelling, ciliary beat, spermatozoa

DOI: 10.3233/BIR-1990-273-435

Journal: Biorheology, vol. 27, no. 3-4, pp. 567-579, 1990