Mechanisms of leukocyte adhesion

Issue title: Proceedings of the Seventh International Congress of Biorheology. Part I. Palais des Congrès, Nancy, France, 18–23 June 1989. Dedicated to Richard Skalak

Guest editors: Alfred L. Copley and Jean-François Stoltz

Article type: Research Article

Authors: Mège, Jean-Louis | Capo, Christian | André, Pascale | Benoliel, Anne-Marie | Bongrand, Pierre

Affiliations: Laboratoire d’Immunologie, Hôpital de Sainte-Marguerite, 13009 Marseille, France

Note: [] Accepted by: Editor G.W. Schmid-Schönbein

Abstract: The interaction between granulocytes and endothelial walls may be influenced by the blood flow. This possibility was investigated by studying the influence of fluid flow on the adhesion and detachment of 51Cr-labeled rat granulocytes interacting with protein-coated glass surfaces. It is concluded that: i)Adhesion is markedly decreased when the wall shear rate becomes higher than about 20 s−1.ii)Pretreating glass with concanavalin A or polylysine significantly decreased adhesion, whereas fibronectin had little effect on binding.iii)Very high flow rates (about one thousandfold higher than those compatible with bond formation) were required to provoke substantial detachment of substrate-bound cells.iv)Coating glass with laminin or polylysine decreased binding strength whereas fibronectin or concanavalin A did not substantially influence this parameter.v)Exposing granulocytes to phorbol myristate acetate might increase the cell ability to form strong adhesions, whereas labile adhesion was unaffected or even decreased by this treatment.

Keywords: Adhesion, Granulocyte, Flow

DOI: 10.3233/BIR-1990-273-420

Journal: Biorheology, vol. 27, no. 3-4, pp. 433-444, 1990