Affiliations: [a] Dept. Haemorheology and Haemodynamics, Institute of Medical Physics and Biophysics, Charite, Humboldt-University, Berlin, German Democratic Republic | [b] Lab. Biochemistry, Frumkin Institute of Electrochemistry, Academy Nauk, Moscow, USSR
Note: [] Accepted by: V.A. Parsegian
Abstract: Under conditions of high pressure difference micropipette aspiration experiments show a “pinch-off” phenomenon as observed by Rand (Mechanical properties of the red cell membrane: II. Viscoelastic breakdown of the membrane. Biophys. J. 4, 303–316, 1964). In a very recent paper, Meier et. al (Fission and refusion of red blood cells using a micropipette technique. Biorheology 24, 287–296, 1987) have reported about new data obtained with a modern electronic technique under moderate mechanical load. We have theoretically analysed the conditions necessary to form such a instability. In our approximations we have investigated the effective transmembrane pressure difference which is given both by the true pressure drop, determined by the hydrodynamic flow through a small gap between the cell tongue and the pipette wall and the interaction between the tongue and the wall, as well as by the nonisotropic part of the membrane tension. When the membrane tongue is long enough (> 3Rp) we have found a condition for the loss of membrane stability in a point where a minimum of the effective transmembrane pressure drop occurs. An expression for it and the vesicle size are given.
