Affiliations: [a] Section of Physiology, Research Institute of Applied Electricity, Hokkaido University, 060 Sapporo, Japan | [b] Laboratory of Pathology, Cancer Institute, School of Medicine, Hokkaido University, 060 Sapporo, Japan
Note: [] Accepted by: Editor E. Fukada
Abstract: In an effort to find a method to intensify the rheological destruction of tumor cells, rat fibrosarcoma KMT 17 cells in ascitic form were exposed to rheological stresses in test solutions; modified Eagle medium with EDTA, and phosphate buffer solution without divalent cations or with verapamil. KMT 17 cells were exposed to a uniform shear stress produced by the rotation of a rotating cone plate viscometer for 1 to 2 hours and to the strong deformation by the passage through Nuclepores of 10, 8 and 5 micrometer. KMT 17 cells suspended in the test solutions were more effectively destroyed by the stresses than those suspended in normal solutions containing divalent cations without any other calcium-suppressing agents. These results suggest that the elimination of divalent cations and/or the block of calcium ion channels of cell membranes intensify the rheological destruction of tumor cells migrating in the circulatory system.
Keywords: Cancer cells, Rheological destruction, divalent cations, EDTA
DOI: 10.3233/BIR-1987-24628
Journal: Biorheology, vol. 24, no. 6, pp. 775-782, 1987
