Studies of rheologically active biological macromolecules by quasielastic light scattering

Issue title: New Methods in Biorheology. Proceedings of the International Symposium held in Nancy, France, 17–18 August 1983. A Satellite Symposium of the Fifth International Congress of Biorheology

Guest editors: J.F. Stoltz and E. Puchelle

Article type: Research Article

Authors: Steiner, C.A.^{a; *} | Litt, M.^{a; b} | Nossal, R.^c

Affiliations: [a] Department of Chemical Engineering, University of Pennsylvania, Philadelphia, Pennsylvania | [b] Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania | [c] National Institutes of Health, Bethesda, Maryland

Note: [*] Present address: Research Center, Hercules, Inc., Wilmington, Delaware.

Abstract: Variables which describe the structure and rheological behavior of macromolecules in both dilute solutions and gels may be determined using methods of quasielastic laser light scattering (QELS). Such parameters include diffusion coefficients, molecular size such as hydrodynamic radius, and viscoelastic moduli. If the polymer is polydisperse, the distribution of radii may be estimated. Application of the methods is illustrated with data on the size distribution of a tracheal mucin glycoprotein solution as an example of a polydisperse polymer solution of biorheological interest. A scheme for measuring shear moduli of polymer gels using dynamic laser light scattering is illustrated with data on reconstituted fibrin clots.

Keywords: Laser light scattering, mucin structure, gel rheology, fibrin gels

DOI: 10.3233/BIR-1984-23S159

Journal: Biorheology, vol. 23, no. s1, pp. 335-344, 1984