Investigation of human blood platelets with fluorescence spectroscopy

Issue title: New Methods in Biorheology. Proceedings of the International Symposium held in Nancy, France, 17–18 August 1983. A Satellite Symposium of the Fifth International Congress of Biorheology

Guest editors: J.F. Stoltz and E. Puchelle

Article type: Research Article

Authors: Viriot, M.L.^a | Collin, T.^a | Gaillard, S.^b | Stoltz, J.F.^b | Andre, J.C.^a

Affiliations: [a] GRAPP du LA 328 du CNRS, ENSIC - INPL, 1, rue Grandville F, 54042 Nancy Cedex | [b] Groupe d’hémorhéologie, CRTS Brabois F 54500 Vandoeuvre, Nancy

Abstract: Intramolecular excimer formation of dipyrenylpropane was investigated in human blood platelets. The ratio of the emission intensities of excimer to monomer (IE/IM) is sensitive to changes in lipidic membrane structure, induced by external perturbations, such as cholesteryl hemisuccinate introduction and temperature. Lack of reliable “microviscosities” determinations could be due to poor solubility of the probe in aqueous cells suspensions. Results were compared to those obtained with the fluorescence polarization technique.

Keywords: blood platelets, fluorescence spectroscopy

DOI: 10.3233/BIR-1984-23S158

Journal: Biorheology, vol. 23, no. s1, pp. 331-333, 1984