Affiliations: [a] School of Chemical, Biological and Materials Engineering, University of Oklahoma, Norman, OK, USA | [b] Samuel Roberts Noble Electron Microscopy Laboratory, University of Oklahoma, Norman, OK, USA | [c] Institute for Biomedical Engineering, Science and Technology, University of Oklahoma, Norman, OK, USA
Correspondence:
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Corresponding author: Edgar A. O’Rear, School of Chemical, Biological and Materials Engineering, University of Oklahoma, 100 E. Boyd SEC T301, Norman, OK 73019, USA. Tel.: +1 405 325 5811; E-mail: [email protected]
Abstract: BACKGROUND:Microparticles (MPs) have activity in thrombus promotion and generation. Erythrocyte microparticles (ErMPs) have been reported to accelerate fibrinolysis in the absence of permeation. We hypothesized that shear induced ErMPs would affect fibrin structure of clots and change flow with implications for fibrinolysis. OBJECTIVE:To determine the effect of ErMPs on clot structure and fibrinolysis. METHODS:Plasma with elevated ErMPs was isolated from whole blood or from washed red blood cells (RBCs) resuspended in platelet free plasma (PFP) after high shear. Dynamic light scattering (DLS) provided size distribution of ErMPs from sheared samples and unsheared PFP controls. Clots were formed by recalcification for flow/lysis experiments and examined by confocal microscopy and SEM. Flow rates through clots and time-to-lysis were recorded. A cellular automata model showed the effect of ErMPs on fibrin polymerization and clot structure. RESULTS:Coverage of fibrin increased by 41% in clots formed from plasma of sheared RBCs in PFP over controls. Flow rate decreased by 46.7% under a pressure gradient of 10 mmHg/cm with reduction in time to lysis from 5.7 ± 0.7 min to 12.2 ± 1.1 min (p < 0.01). Particle size of ErMPs from sheared samples (200 nm) was comparable to endogenous microparticles. CONCLUSIONS:ErMPs alter the fibrin network in a thrombus and affect hydraulic permeability resulting in decelerated delivery of fibrinolytic drugs.
