Affiliations: Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, USA | Department of Pathology & Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA | Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA
Note: [] Address for correspondence: Peter F. Davies, Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Research Labs, 3340 Smith Walk, Philadelphia, PA 19104‐6383, USA. Tel.: +1 215 573 6813; Fax: +1 215 573 6815; E‐mail: [email protected].
Note: [] Dr. Helmke's present address is: the Department of Biomedical Engineering, University of Virginia, Charlottesville, USA.
Abstract: The completion of the Human Genome Project and ongoing sequencing of mouse, rat and other genomes has led to an explosion of genetics‐related technologies that are finding their way into all areas of biological research; the field of biorheology is no exception. Here we outline how two disparate modern molecular techniques, microarray analyses of gene expression and real‐time spatial imaging of living cell structures, are being utilized in studies of endothelial mechanotransduction associated with controlled shear stress in vitro and haemodynamics in vivo. We emphasize the value of such techniques as components of an integrated understanding of vascular rheology. In mechanotransduction, a systems approach is recommended that encompasses fluid dynamics, cell biomechanics, live cell imaging, and the biochemical, cell biology and molecular biology methods that now encompass genomics. Microarrays are a useful and powerful tool for such integration by identifying simultaneous changes in the expression of many genes associated with interconnecting mechanoresponsive cellular pathways.
Journal: Biorheology, vol. 39, no. 3-4, pp. 299-306, 2002
