Affiliations: [a] Department of Physiology, School of Medicine, Ehime University, Shigenobu, Onsen-gun, Ehime, Japan 791-02 | [b] Central Research Laboratories, School of Medicine, Ehime University, Shigenobu, Onsen-gun, Ehime, Japan 791-02 | [c] Laboratory of Biology, Hitotsubashi University, Kunitachi, Tokyo, Japan 186
Note: [1] Paper prepared at the 4th International Congress of Biorheology, Tokyo, 1981.
Note: [] Accepted by: Guest Editors N. Ohshima and D.E. Brooks
Abstract: The effect of two cationic drugs (chlorpromazine and isoxsuprine) 011 the suspension viscosity of human erythrocytes were examined, comparing with the effect of anionic drugs.(1)As increasing the drug concentrations, the cationic drugs transformed the erythrocytes to stomatocytes, then to spherostomatocytes, while trinitrobenzene sulfonate, dehydroepiandrosterone sulfate and lysolecithin induced echinocytes, as well known.(2)The suspension viscosity decreased in parallel with the appearance of spherostomatocytes, but it increased in echinocytosis.(3)The membrane fluidity, measured by spin label method, was not a major determinant for the suspension viscosity in these cases, because of no systematic correlation.(4)The rheoscopic observation under shear force demonstrated that the spherostomatocytes deformed easily to ellipsoid with smooth cell surface, while the echinocytes less easily deformed to ellipsoid on which the small spikes persisted at higher shear. These distinct difference in deformed shape under high shear force could be related to the decreased suspension viscosity of spherostomatocytes.(5)In addition, the transformation to spherostomatocytes, thus the decreased viscosity, was primarily determined by the intramembraneous drug concentration.
