Affiliations: Erasmus University Medical Centre Rotterdam, Department of Orthopaedics, Rotterdam, The Netherlands | Erasmus University Medical Centre Rotterdam, Department of Otorhinolaryngology, Rotterdam, The Netherlands
Note: [] Address for correspondence: Gerjo J.V.M. van Osch, Dept. Orthopaedics and Otorhinolaryngology, Erasmus University Rotterdam, Room Ee1659, PO Box 1738, 3000 DR Rotterdam, The Netherlands. Tel.: +31 10 4087661; Fax: +31 10 4089441; E‐mail: [email protected].
Abstract: Tissue engineering of cartilage consists of two steps. Firstly, the cells from a small biopsy of patient's own tissue have to be multiplied. During this multiplication process they lose their cartilage phenotype. In the second step, these cells have to be stimulated to re‐express their cartilage phenotype and produce cartilage matrix. Growth factors can be used to improve cell multiplication, redifferentiation and production of matrix. The choice of growth factors should be made for each phase of the tissue engineering process separately, taking into account cell phenotype and the presence of extracellular matrix. This paper demonstrates some examples of the use of growth factors to increase the amount, the quality and the assembly of the matrix components produced for cartilage tissue engineering. In addition it shows that the “culture history” (e.g., addition of growth factors during cell multiplication or preculture period in a 3‐dimensional environment) of the cells influences the effect of growth factor addition. The data demonstrate the potency as well as the limitations of the use of growth factors in cartilage tissue engineering.
Journal: Biorheology, vol. 39, no. 1-2, pp. 215-220, 2002
