Affiliations: Laboratoire d'Angiohématologie‐Hémorhéologie, UMR CNRS 7563 (LEMTA), Faculté de Médecine, 54500 Vandoeuvre lès Nancy Cedex, France | Laboratoire de Physico‐Chimie des Colloïdes, UMR CNRS 7565 (SRSMC), Faculté des Sciences, Université Henri Poincaré, BP 239, 54506 Vandoeuvre lès Nancy Cedex, France
Note: [] Address for correspondence: Dr. P. Mazeron, Laboratoire de Physico‐chimie des Colloïdes, UMR CNRS 7565 (SRSMC), Faculté des Sciences, Université Henri Poincaré, BP 239, 54506 Vandoeuvre lès Nancy Cedex, France. Fax: +33 383 91 25 32; E‐mail: [email protected]‐nancy.fr.
Abstract: The theoretical extinction of blood suspensions submitted to a slow dialysis is analyzed in terms of their NaCl concentration. The model involves two adjustable parameters, χ and K, related to swelling and hemolysis. During swelling, the erythrocyte volume is supposed to vary linearly with the saline concentration. During hemolysis, an exponential decay of the hemoglobin concentration in the erythrocyte is used. The theoretical transmittance curves are consistent with the measurements carried out at a wavelength of 0.808 μm on native and incubated blood samples. χ and K are relevant parameters to characterize quantitatively the fragility of the erythrocyte membrane. The effect of a non ideal character of the hemoglobin solutions and of normal distributions of χ and K is also discussed.
