New techniques to detect oxidative stress markers: Mass
spectrometry-based methods to detect isoprostanes as the gold standard for
oxidative stress in vivo
Issue title: Papers from the Workshop on Markers of
Oxidative Stress and Degenerative Diseases, October 13, 2008, Taiwan
Affiliations: Division of Clinical Pharmacology, Departments of
Medicine, Pharmacology, and Chemistry, Vanderbilt University School of
Medicine, Nashville, TN, USA
Note: [] Address for correspondence: Huiyong Yin, Ph.D., Division of
Clinical Pharmacology, Departments of Medicine, Pharmacology, and Chemistry,
Vanderbilt University School of Medicine, 23rd and Pierce Aves, Nashville, TN
37232-6602, USA. Tel.: +1 615/322 6569; Fax: +1 615/322 3669; E-mail:
[email protected]
Abstract: Free radical-induced lipid oxidation under oxidative stress has been
implicated in a number of human diseases. Isoprostanes (IsoPs), isomers of
prostaglandins, are one of the major classes of oxidation products derived from
this oxidation process. Measurement of the levels of IsoPs by Mass
Spectrometry-based methods has become the "gold standard" biomarker of
oxidative stress in vivo. Significant advances have been made in
understanding this important pathway of lipid peroxidation since the discovery
of IsoP formation in vivo 18 years ago. Studies from our laboratory and
others are discussed that have provided insights into the mechanism of
formation. Furthermore, new independent studies have demonstrated that IsoPs
are the most reliable available marker of lipid peroxidation in vivo, and
recent work examining IsoP formation has provided valuable information about
the pathogenesis of numerous human diseases. Thus, the complexity of the IsoP
pathway has expanded, providing novel insights into mechanisms of lipid
peroxidation in vivo and allowing investigators to explore the role of
oxidative stress in human disease.
