Affiliations: School of Life Sciences, Devi Ahilya University,
Takhshila Campus, Indore, India
Note: [] Address for correspondence: Dr. (Ms) Sunanda Panda, School of
Life Sciences, Devi Ahilya University, Takshashila Campus, Khandwa Road,
Indore-452017, Madhya Pradesh, India. Tel.: +91 731 2477166; E-mail:
[email protected]
Abstract: Present investigation was made to reveal the involvement of a
quercetin in the antidiabetic and antiperoxidative effects of Annona
squamosa leaf extract. Quercetin-3-O-glucoside (characterized by UV, IR, MS and NMR
analyses) was isolated from Annona squamosa leaves and examined for its
potential to regulate alloxan-induced hyperglycemia and lipid peroxidation
(LPO) in rats. While in alloxan treated animals, an increase in the
concentration of serum glucose with a parallel decrease in insulin level was
observed, administration of 15 mg/kg/day of isolated quercetin-3-O-glucoside
for 10 consecutive days to the hyperglycemic animals reversed these effects and
simultaneously inhibited the activity of hepatic glucose-6-phosphatase. It
further decreased the hepatic and renal LPO with a concomitant increase in the
activities of antioxidative enzymes, such as catalase (CAT) and superoxide
dismutase (SOD) and in glutathione (GSH) content, indicating its safe and
antiperoxidative effects. These findings suggest the potential of
quercetin-3-O-glucoside in the amelioration of diabetes mellitus and tissue
lipid peroxidation. It also appears that the antidiabetic effects of A.
squamosa leaf extract is possibly mediated through the insulin stimulating
and/or free radical scavenging properties of its active constituent,
quercetin-3-O-glucoside.
