Affiliations: Research and Development Department, Mitsubishi Kagaku
Bio-Clinical Laboratories, Inc., Tokyo, Japan | New Business Development Department, Mitsubishi Kagaku
Bio-Clinical Laboratories, Inc., Tokyo, Japan
Note: [] Address for correspondence: Kyouichi Sekine, Research and
Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc.
3-30-1 Shimura, Itabashi-ku, Tokyo, 174-8555, Japan. Tel.: +81 3 5994 2413;
Fax: +81 3 5994 2917; E-mail: [email protected]
Abstract: Coenzyme Q10 (CoQ_{10}) levels in human saliva
were measured by HPLC with a highly sensitive electrochemical detector (ECD)
and a special concentration column. This HPLC system showed satisfactory
analytical results within the standard range of 0.78–50 ng/ml. We also
found a significant correlation between CoQ_{10} levels in
plasma and in saliva from parotid glands, while this correlation was lacking
between plasma CoQ10 and CoQ10 in whole saliva. Unlike in plasma, there are
some fluctuations of saliva CoQ_{10} levels throughout the
day. A good correlation was obtained by colleting parotid gland saliva at times
between meals. The mean saliva CoQ_{10} level for 55 healthy
volunteers was 17.0 ng/ml (S.D. 6.8 ng/ml); approximately one fiftieth of that
in plasma. Regarding the influence of oral supplementation,
CoQ_{10} was analyzed in plasma and parotid gland saliva
from 20 healthy volunteers supplemented daily with 100 mg of
CoQ_{10} for the first week and 200 mg for the second. The
plasma CoQ_{10} levels of all volunteers increased to
different extents in accordance with the CoQ_{10} daily
intake and the corresponding change in saliva showed almost the same trend.
