Molecular effects of fermented papaya preparation on oxidative damage, MAP Kinase activation and modulation of the benzo[a]pyrene mediated genotoxicity

Issue title: Food Factors

Article type: Research Article

Authors: Aruoma, Okezie I.^; | Colognato, Renato | Fontana, Ilaria | Gartlon, Joanne | Migliore, Lucia | Koike, Keiko | Coecke, Sandra | Lamy, Evelyn | Mersch-Sundermann, Volker | Laurenza, Incoronata | Benzi, Luca | Yoshino, Fumihiko | Kobayashi, Kyo | Lee, Masaichi-Chang-il

Affiliations: Faculty of Health and Social Care, London South Bank University, 103 Borough Road, London, UK | Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University, Seoul, Korea | Università di Pisa, Dip. Scienze dell'Uomo e dell'Ambiente, Sezione di Genetica, Pisa, Italy | Osato Research Institutes, Gifu, Japan | The J.R.C.- Ispra, ECVAM Unit TP 580, Ispra (VA), Italy | Institute of Indoor and Environmental Toxicology, Faculty of Medicine, Justus-Liebig University of Giessen, Giessen, Germany | Dip. di Endocrinoliga, Metabolismo Traumatologia e Medicina del Lavoro, Pisa, Italy | Department of Clinical Care Medicine, Division of Pharmacology & ESR Laboratories, Kanagawa Dental College, Kanagawa, Japan

Note: [] Address for correspondence: Okezie I. Aruoma, Faculty of Health and Social Care, London South Bank University, 103 Borough Road, London SE1 0AA, UK. E-mail: [email protected]; [email protected]

Abstract: The involvement of oxidative and nitrosative stress mechanisms in several biological and pathological processes including aging, cancer, cardiovascular and neurodegenerative diseases has continued to fuel suggestions that processes can potentially be modulated by treatment with free-radical scavengers and antioxidant. The fermented papaya preparation (FPP) derived from Carica papaya Linn was investigated for its ability to modulate oxidative DNA damage due to H_2O_2 in rat pheochromocytoma (PC12) cells and protection of brain oxidative damage in hypertensive rats. Cells pre-treated with FPP (50 μg/ml) prior to incubation with H_2O_2 had significantly increased viability and sustenance of morphology and shape. The human hepatoma (HepG2) cells exposed to H_2O_2 (50 μM) showed an olive tail moment of 10.56 ± 1.44 compared to 1.37 ± 0.29 of the solvent control. A significant reduction (P ⩽ 0.05) of DNA damage was observed at concentrations ⩾ 10 μg/ml FPP, with 50 μg/ml FPP reducing the genotoxic effect of H_2O_2 by about 1.5-fold compared to only H_2O_2 exposed cells.

Journal: BioFactors, vol. 26, no. 2, pp. 147-159, 2006