Ubiquinol and the papaverine derivative caroverine prevent the expression of tumour- promoting factors in adenoma and carcinoma colon cancer cells induced by dietary fat

Issue title: The Fourth Conference of the International CoQ10 Association

Article type: Research Article

Authors: Nohl, Hans | Rohr-Udilova, Natalia | Gille, Lars | Bieberschulte, Werner | Jurek, Daniela | Marian, Brigitte | Schulte-Hermann, Rolf

Affiliations: Research Institute of Biochemical Pharmacology and Toxicology, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210 Vienna, Austria | PHAFAG AG, FL-9486, Schaanwald, Liechtenstein | Institute of Cancer Research, University of Vienna, A-1090 Vienna, Austria

Note: [] Corresponding author. Fax: +43 1 25077 4490; E-mail: [email protected]

Abstract: High consumption of dietary fat promotes colon carcinogenesis. While this effect is well known the underlying mechanism is not understood. Fatty acid hydroperoxides (LOOH) arise from unsaturated fatty acids in the presence of oxygen and elevated temperature during food processing. An approach was made starting from the assumption that LOOH are present in dietary fats as a result of boiling. LOOH undergoes homolytic cleavage in the presence of iron. We studied their effects on gene expression in colorectal tumour cells using linoleic acid hydroperoxide (LOOH) as model compound. Addition to the medium of LT97 adenoma and SW480 carcinoma cells enhanced the production of hydrogen peroxide. Both cell lines were observed to increase VEGF and COX-II expression based on mRNA. Expression of VEGF was inhibited by caroverine and ubiquinon.

Journal: BioFactors, vol. 25, no. 1-4, pp. 87-95, 2005