Affiliations: National Institute of Advanced Industrial Science and
Technology (AIST), Human Stress Signal Research Center, Ikeda 563-8577,
Japan
Note: [] Address for correspondence: Yasukazu Yoshida, AIST, Human Stress
Signal Research Center, 1-8-31 Midorigaoka, Ikeda 563-8577, Japan. Tel.: +81 72
751 8183; Fax: +81 72 751 9964; E-mail: [email protected]
Abstract: An improved method for the measurement of lipid peroxidation in
vivo has been recently developed, where total hydroxyoctadecadienoic acid
(HODE) and 7-hydroxycholesterol (FCOH) were determined by GC/MS analysis from
physiological samples after reduction with sodium borohydride and
saponification by potassium hydroxide. In this method, both free and ester
forms of hydroperoxides and ketones as well as hydroxides of linoleic acid and
cholesterol are measured as HODE and FCOH, respectively. The ratio of
stereo-isomer, (Z, E)-HODE/(E, E)-HODE, could be also measured. In the present
study, in order to examine the effect of continuous, slow flux of free radicals
in vivo, a water-soluble radical generator was administered to rats and
mice and the amounts of HODE and 8-isoprostane in plasma and liver were
measured. It was found that the administration of free radical-generating azo
compound increased the level of HODE and decreased the (Z, E)-HODE/(E, E)-HODE
ratio in both plasma and liver. The level of HODE was much higher than
8-isoprostane.
