Affiliations: Research Institute for Environmental Medicine (IUF),
Heinrich-Heine-University, Duesseldorf, Germany
Note: [] Address for correspondence: Tilman Grune, Research Institute of
Environmental Medicine, Heinrich Heine University, Auf'm Hennekamp 50, 40225
Düsseldorf, Germany. Fax: +49 211 3389 222; E-mail:
[email protected]
Abstract: Protein modification is one of the important processes during
oxidative stress. This modification of proteins is either due to direct
oxidation of proteins by various oxidants or due to secondary modification by
lipid peroxidation products, e.g. 4-hydroxynonenal. In the here presented work
we compare the intracellular distribution of protein modification products
after treatment of human U87 astrocytoma cells with hydrogen peroxide or HNE.
The treatment with hydrogen peroxide leads mainly to a cytosolic formation of
oxidized proteins whereas HNE treatment is forming HNE-adducts throughout the
cell. Therefore, we concluded that HNE diffusion distance in cells enables
this lipid peroxidation product to act as a second messenger within the cell
and on the other hand is the reason for the genotoxic properties of this
compound.
Keywords: Oxidative stress, protein carbonyls, HNE, protein oxidation
Journal: BioFactors, vol. 24, no. 1-4, pp. 165-170, 2005
